Multi-contrast in vivo optical tomography

Speaker：

Wuwei Ren 任无畏

ShanghaiTech University

Time：

Tuesday, 10:00-11:00 am

August 19, 2025

Venue：

C654, Shuangqing Complex Building

Abstract:

Multi-contrast imaging technology is of great value in biomedical research and clinical diagnosis. Taking magnetic resonance imaging (MRI) as an example, the multi-contrast data generated by its multi-sequence scanning can meet the diverse clinical diagnostic needs through the complementary integration of structural and functional information. In the field of optical imaging, multi-contrast technology can not only enhance the data dimension, but also break through the bottleneck of quantification: for example, the deviation of optical absorption/scattering coefficients in fluorescence imaging will significantly affect the three-dimensional quantitative reconstruction of fluorescent probes, and the multi-contrast strategy of synchronously acquiring optical coefficients and fluorescence distribution can effectively solve this problem. This study integrates structured light scanning, single-photon avalanche diode (SPAD) detectors and pulsed laser modulation technology to construct a multi-contrast optical tomography system (SHMOT). The system can simultaneously achieve high-precision reconstruction of tissue surface, three-dimensional analysis of optical absorption/scattering coefficients, and visualization of quantitative distribution of exogenous fluorescent probes. After the reliability of the system was verified by digital simulation and phantom experiments, SHMOT has been successfully applied to in vivo tumor microenvironment imaging and dynamic monitoring of thermal ablation temperature field in mice. This technology shows clear clinical application potential in the fields of precise disease diagnosis (such as tumor boundary identification) and fluorescence-guided surgical navigation.